cck8 reagent  (TargetMol)

Journal: Cancer Cell International

Article Title: PIBF1 (p.R405Q) germline variant identified in cancer susceptibility family impairs protein stability and function

doi: 10.1186/s12935-025-04132-y

Figure Lengend Snippet: PIBF1 (p.R405Q) mutation is a candidate gene for hereditary cancer syndromes. A . The expression of PIBF1 is low in BRCA patients in the UALCAN database. B . The expression level of PIBF1 in the UALCAN database is negatively correlated with the tumor grade. C . The Kaplan-Meier Plotter plots survival curves of PIBF1 and low expression of PIBF1 was correlated with poor prognosis of breast cancer. D , E . Q-PCR and WB detection of PIBF1-WT and PIBF1(p.R405Q) in the MDA-MB-231 and MDA-MB-468. F . CCK8 was performed to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the cell growth of breast cancer cells. G , H . Plate cloning assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the proliferation of breast cancer cells. I . Transwell assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the invasion ability of breast cancer cells. J . WB assay to detect the regulatory effect of PIBF1-WT and PIBF1(p.R405Q) on ERK phosphorylation. All data are presented as mean ± SEM ( n = 3 independent cell culture experiments), *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: The medium was refreshed every 24 h with a 1:10 dilution of CCK8 reagent (Cat#C0005, TargetMol, USA) and incubated at 37 °C for 3 hours before measuring absorbance at 450 nm using a microplate reader (EnSpire 2300, PerkinElmer, USA).

Techniques: Mutagenesis, Expressing, Cloning, Transwell Assay, Phospho-proteomics, Cell Culture

Journal: European Journal of Medical Research

Article Title: The roles of both the endogenous synthesis and exogenous uptake of fatty acids in thyroid cancer cell proliferation

doi: 10.1186/s40001-025-03582-4

Figure Lengend Snippet: LPL regulates tumour progression in PTC cell lines. A , B The total protein and mRNA levels of LPL in K1 and BCPAP cells transfected with si-LPL1 or si-LPL2 were detected by Western blot ( A ) and RT-qPCR ( B ) ( n = 3). C CCK8 assay results showing that the downregulation of LPL can inhibit the proliferation of TC cells ( n = 3). D Colony formation assays revealed changes in the proliferative capacity of si-LPL- or si-NC- transfected PTC cells ( n = 3). The downregulation of LPL can inhibit the colony formation of PTC cells. E TUNEL staining revealed that the downregulation of LPL promoted the apoptosis of PTC cells ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. si-nc. LPL lipoprotein lipase, PTC papillary thyroid carcinoma

Article Snippet: From Day 1 to Day 3, 10 μl of Cell Counting Kit-8 (CCK8) reagent (cat. no. HY-K0301; MedChemExpress, Inc.) was added to each well.

Techniques: Transfection, Western Blot, Quantitative RT-PCR, CCK-8 Assay, TUNEL Assay, Staining

Journal: European Journal of Medical Research

Article Title: The roles of both the endogenous synthesis and exogenous uptake of fatty acids in thyroid cancer cell proliferation

doi: 10.1186/s40001-025-03582-4

Figure Lengend Snippet: FASN regulates tumour progression in PTC cell lines. A The protein levels of FASN in PTC (K1 and BCPAP) cells and normal thyroid cells (N-3-1). FASN is highly expressed in PTC cell lines. B After C75, a specific FASN inhibitor, was used, Western blotting analysis confirmed the effective downregulation of FASN protein expression in PTC cells. C CCK8 assay results demonstrated that suppression of FASN effectively inhibited the proliferation of PTC cells ( n = 3). D Colony formation assay results revealed that suppression of FASN can inhibit the colony formation of PTC cells ( n = 3). E TUNEL staining revealed that suppression of FASN promoted the apoptosis of PTC cells ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the control. FASN fatty acid synthase, PTC papillary thyroid carcinoma

Article Snippet: From Day 1 to Day 3, 10 μl of Cell Counting Kit-8 (CCK8) reagent (cat. no. HY-K0301; MedChemExpress, Inc.) was added to each well.

Techniques: Western Blot, Expressing, CCK-8 Assay, Colony Assay, TUNEL Assay, Staining, Control

Journal: European Journal of Medical Research

Article Title: The roles of both the endogenous synthesis and exogenous uptake of fatty acids in thyroid cancer cell proliferation

doi: 10.1186/s40001-025-03582-4

Figure Lengend Snippet: Combined downregulation of LPL and FASN inhibits PTC cell proliferation and boosts apoptosis. Simultaneous treatment with the pharmacological inhibitor C75 of FASN and siRNA-mediated downregulation of LPL resulted in the suppression of both FASN and LPL expression. A Western blot analysis was conducted to detect the protein levels of LPL in K1 and BCPAP cells following C75 inhibition. These results indicated that C75 had no significant impact on LPL expression. B CCK8 assay results revealed that the combined downregulation of LPL and FASN has a synergistic effect, further inhibiting the proliferation of PTC cells ( n = 3). Statistical analysis revealed significant between-group differences ( P < 0.05) exclusively at the 72-h time point; consequently, the graph presents only these time-specific results. C Colony formation assay results indicated that the simultaneous downregulation of LPL and FASN significantly diminished the clonogenic capacity of PTC cells ( n = 3). E TUNEL staining revealed that the combined downregulation of LPL and FASN further promoted the apoptosis of BCPAP cells ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. si-LPL + C75. LPL lipoprotein lipase, FASN fatty acid synthase, PTC papillary thyroid carcinoma

Article Snippet: From Day 1 to Day 3, 10 μl of Cell Counting Kit-8 (CCK8) reagent (cat. no. HY-K0301; MedChemExpress, Inc.) was added to each well.

Techniques: Expressing, Western Blot, Inhibition, CCK-8 Assay, Colony Assay, TUNEL Assay, Staining