cck8 reagent  (TargetMol)

Journal: European Journal of Medical Research

Article Title: The roles of both the endogenous synthesis and exogenous uptake of fatty acids in thyroid cancer cell proliferation

doi: 10.1186/s40001-025-03582-4

Figure Lengend Snippet: LPL regulates tumour progression in PTC cell lines. A , B The total protein and mRNA levels of LPL in K1 and BCPAP cells transfected with si-LPL1 or si-LPL2 were detected by Western blot ( A ) and RT-qPCR ( B ) ( n = 3). C CCK8 assay results showing that the downregulation of LPL can inhibit the proliferation of TC cells ( n = 3). D Colony formation assays revealed changes in the proliferative capacity of si-LPL- or si-NC- transfected PTC cells ( n = 3). The downregulation of LPL can inhibit the colony formation of PTC cells. E TUNEL staining revealed that the downregulation of LPL promoted the apoptosis of PTC cells ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. si-nc. LPL lipoprotein lipase, PTC papillary thyroid carcinoma

Article Snippet: From Day 1 to Day 3, 10 μl of Cell Counting Kit-8 (CCK8) reagent (cat. no. HY-K0301; MedChemExpress, Inc.) was added to each well.

Techniques: Transfection, Western Blot, Quantitative RT-PCR, CCK-8 Assay, TUNEL Assay, Staining

Journal: European Journal of Medical Research

Article Title: The roles of both the endogenous synthesis and exogenous uptake of fatty acids in thyroid cancer cell proliferation

doi: 10.1186/s40001-025-03582-4

Figure Lengend Snippet: FASN regulates tumour progression in PTC cell lines. A The protein levels of FASN in PTC (K1 and BCPAP) cells and normal thyroid cells (N-3-1). FASN is highly expressed in PTC cell lines. B After C75, a specific FASN inhibitor, was used, Western blotting analysis confirmed the effective downregulation of FASN protein expression in PTC cells. C CCK8 assay results demonstrated that suppression of FASN effectively inhibited the proliferation of PTC cells ( n = 3). D Colony formation assay results revealed that suppression of FASN can inhibit the colony formation of PTC cells ( n = 3). E TUNEL staining revealed that suppression of FASN promoted the apoptosis of PTC cells ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the control. FASN fatty acid synthase, PTC papillary thyroid carcinoma

Article Snippet: From Day 1 to Day 3, 10 μl of Cell Counting Kit-8 (CCK8) reagent (cat. no. HY-K0301; MedChemExpress, Inc.) was added to each well.

Techniques: Western Blot, Expressing, CCK-8 Assay, Colony Assay, TUNEL Assay, Staining, Control

Journal: European Journal of Medical Research

Article Title: The roles of both the endogenous synthesis and exogenous uptake of fatty acids in thyroid cancer cell proliferation

doi: 10.1186/s40001-025-03582-4

Figure Lengend Snippet: Combined downregulation of LPL and FASN inhibits PTC cell proliferation and boosts apoptosis. Simultaneous treatment with the pharmacological inhibitor C75 of FASN and siRNA-mediated downregulation of LPL resulted in the suppression of both FASN and LPL expression. A Western blot analysis was conducted to detect the protein levels of LPL in K1 and BCPAP cells following C75 inhibition. These results indicated that C75 had no significant impact on LPL expression. B CCK8 assay results revealed that the combined downregulation of LPL and FASN has a synergistic effect, further inhibiting the proliferation of PTC cells ( n = 3). Statistical analysis revealed significant between-group differences ( P < 0.05) exclusively at the 72-h time point; consequently, the graph presents only these time-specific results. C Colony formation assay results indicated that the simultaneous downregulation of LPL and FASN significantly diminished the clonogenic capacity of PTC cells ( n = 3). E TUNEL staining revealed that the combined downregulation of LPL and FASN further promoted the apoptosis of BCPAP cells ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. si-LPL + C75. LPL lipoprotein lipase, FASN fatty acid synthase, PTC papillary thyroid carcinoma

Article Snippet: From Day 1 to Day 3, 10 μl of Cell Counting Kit-8 (CCK8) reagent (cat. no. HY-K0301; MedChemExpress, Inc.) was added to each well.

Techniques: Expressing, Western Blot, Inhibition, CCK-8 Assay, Colony Assay, TUNEL Assay, Staining

Journal: Translational Oncology

Article Title: Novel multi-omics analysis revealing metabolic heterogeneity of breast cancer cell and subsequent development of associated prognostic signature

doi: 10.1016/j.tranon.2025.102444

Figure Lengend Snippet: PDCD1 knockdown alters proliferation, invasion, and migration in MCF7. a qPCR validation of PDCD1 knockdown efficiency following siRNA transfection in MCF7. b CCK8 assay demonstrating a significant reduction in cell proliferation following PDCD1 knockdown compared to the control group. c Transwell assay results showing a marked decrease in the invasive capacity ofMCF7 upon PDCD1 knockdown relative to the control group. d Wound healing assay illustrating a notable reduction in wound closure ability in PDCD1 knockdown cells compared to the control group. e EDU assay displaying a significant decrease in fluorescence intensity following PDCD1 knockdown, indicating reduced cell proliferation. * p < 0.001 for all comparisons, *** p < 0.0001.

Article Snippet: To ensure experimental reproducibility, three replicate wells were prepared for each experimental group.According to the manufacturer's instructions, CCK8 reagent (KeyGEN, China) was combined with complete culture medium to ensure a total volume of 200 μl per well, which was rapidly added to the 96-well plate using a pipette.

Techniques: Knockdown, Migration, Biomarker Discovery, Transfection, CCK-8 Assay, Control, Transwell Assay, Wound Healing Assay, EdU Assay, Fluorescence